Antisense Technology

A Practical Approach (Practical Approach Series)

Publisher: Oxford University Press, USA

Written in English
Cover of: Antisense Technology |
Published: Pages: 326 Downloads: 936
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Edition Notes

ContributionsConrad Lichtenstein (Editor), Wolfgang Nellen (Editor)
The Physical Object
Number of Pages326
ID Numbers
Open LibraryOL7406032M
ISBN 100199635838
ISBN 109780199635832

In molecular biology and genetics, the sense of a nucleic acid molecule, particularly of a strand of DNA or RNA, refers to the nature of the roles of the strand and its complement in specifying a sequence of amino ing on the context, sense may have slightly different meanings. For example, DNA is positive-sense if an RNA version of the same sequence is translated or translatable. Antisense inhibition of biotin carboxylase gene in tobacco led to severe retardation of growth, reinforcing the importance of biotin for plant growth (Shintami et al. ). The antisense RNA technology has also been used for crop improvement, besides being used to . Extensively revised and updated, Antisense Drug Technology: Principles, Strategies, and Applications, Second Edition reflects the logarithmic progress made in the past four years of oligonucleotide-based therapies, and, in particular, antisense therapeutics and research. Interpreting lessons learned from the clinical trials of first generation drugs, the book evaluates the technology as a. Antisense technology may result in dramatic changes in the therapy of many diseases and may provide tools to dissect pharmacological processes and to confirm the roles of various genes. In this volume, progress in the understanding of antisense technology and its use in creating new drugs is.

  Applied Antisense Oligonucleotide Technology is an essential reference for researchers in biotechnology, genetics, and molecular biology. Easy to read and thoughtfully compiled by the co-editors of the journal Antisense Research and Development, this work is also accessible to researchers and academics in other areas, and anyone interested in 4/5(1). The only serious criticism is the omission of a chapter on the use of decoy oligonucleotides, which can be used to silence DNA-binding proteins. Despite this small oversight, I would recommend this book as a useful reference source to all scientists who hope to elucidate the functions of specific proteins using antisense by: 1. Part I of the book eases the reader in with reviews of the technology of antisense, its chemistry, and its role in target validation. One of the earliest problems for antisense was the need to Author: Liz Fletcher.   Antisense therapy is an approach to fighting diseases using DNA-like molecules (AOs). After initially observing antisense-mediated RNA regulation in nature, investigations using model systems to test the feasibility of using synthetic AOs to reduce Cited by:

micro RNA In The Mix. Antisense and RNAi are both synthesized in the lab and delivered to patients to decrease the expression of a disease-associated protein. MicroRNA (miR), on the other hand, is a type of dsRNA made by cells to regulate gene expression. Like RNAi, miR is processed by enzymes DICER and RISC into single-stranded RNA capable of binding to disease-associated mRNA with a.   Antisense: In molecular biology, the strand complementary to a coding sequence of a nucleic acid. Antisense DNA is the non-coding strand complementary to the coding strand in double-stranded DNA. The antisense strand serves as the template for messenger RNA (mRNA) synthesis. Antisense RNA is the non-coding strand complementary to a coding sequence of mRNA, a molecule . Following this initial enthusiasm, antisense oligonucleotides have been maligned as nonspecific, toxic, and essentially useless. However, application of antisense oligonucleotide technology in the nervous system stands apart from the use of this technique in peripheral systems, . Advances in Ionis' Novel Antisense Technology to be Presented at Society for Neuroscience Meeting 12 presentations highlighting latest data from Ionis' Neurodegenerative Program including.

Antisense Technology Download PDF EPUB FB2

Extensively revised and updated, Antisense Drug Technology: Principles, Strategies, and Applications, Second Edition reflects the logarithmic progress made in the past four years of oligonucleotide-based therapies, and, in particular, antisense therapeutics and research.

Interpreting lessons learned from the clinical trials of first generation drugs, the book evaluates the technology as a whole and offers new Price: $ "This book is part of a large series of instruction manuals on diverse topics in biochemistry and molecular biology.

The 14 individually authored chapters in this volume contain practices and protocols of interest to the advanced researcher in the field of antisense nucleic acid : Paperback. Purchase Antisense Technology, Part B: Applications, Volume Antisense Technology book 1st Edition.

Print Book & E-Book. ISBNBook Edition: 1. Antisense technology is a powerful procedure that permits the controlled silencing of a specific gene for investigations of mRNA and protein function. This valuable text provides proven step-by-step protocols for antisense techniques in a range of different organisms and cell culture addition it discusses the potential benefits and problems for various antisense methods which.

Book Description. Extensively revised and updated, Antisense Drug Technology: Principles, Strategies, and Applications, Second Edition reflects the logarithmic progress made in the past four years of oligonucleotide-based therapies, and, in particular, antisense therapeutics and research.

Interpreting lessons learned from the clinical trials of first generation drugs, the book evaluates the. Purchase Antisense Technology, Part A, General Methods, Methods of Delivery, and RNA Studies, Volume - 1st Edition.

Print Book & E-Book. ISBNPresents previously unpublished data on the use of antisense technology to dissect pharmacological processes and confirm the roles of various genes. Showcasing the benefits of antisense drug use, Reviews: 1. Antisense Technology Antisense RNA and DNA techniques have been developed as a relatively recent approach to the specific modulation of gene expression in vitro and in vivo.

The purpose of this. Antisense technology can be employed to modulate RNA metabolism and expression through several mechanisms (Crooke,and Table 1) including the modulation of RNA splicing (Condon and Bennett, ), the inhibition of mRNA translation into protein (Alt et al., ; Baker, ; Brown-Driver et al., ; Zhang et al., ) the disruption of necessary RNA structures regulating RNA.

Therapeutic Application of Antisense Technology. A second application of this technology, and one that is potentially of more immediate relevance to the practicing physician, is the use of Antisense Technology book technology in therapy ().In principle, antisense oligonucleotides complementary to viral RNAs can suppress a wide variety of viral infections; a tremendous amount of research is ongoing in this by: 2.

Antisense therapy is a form of treatment for genetic disorders or infections. When the genetic sequence of a particular gene is known to cause a particular disease, it is possible to synthesize a strand of nucleic acid (DNA, RNA or a chemical analogue) that will bind to the messenger RNA (mRNA) produced by that gene and inactivate it, effectively turning that gene "off".

Book Review of Antisense Drug Technology: Principles, Strategies, and Applications. Second Edition. Gary EvansAuthor: Gary Evans.

antisense technology Download antisense technology or read online books in PDF, EPUB, Tuebl, and Mobi Format. Click Download or Read Online button to get antisense technology book now. This site is like a library, Use search box in the widget to get ebook that you want. Antisense inhibitors continue to mature as integral research tools for understanding biological mechanisms, and have made great progress as therapeutic agents since the last comprehensive reviews in this area were published [1].Cited by:   Abstract.

Antisense reagents and technology have developed as extraordinarily useful tools for the analysis of gene function. The capacity of antisense to reduce expression of RNA (including protein-encoding mRNA and noncoding RNA) important in a multitude of diseases has led to the concept of using antisense molecules as drugs to treat those by: 4.

Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation.

For example, methods that inhibit gene expression permit studies which probe. Antisense Therapeutics Limited () is an Australian publicly traded biopharmaceutical drug discovery and development company whose mission is to create, develop and commercialize novel antisense therapeutics for a variety of drug candidates including Duchenne Muscular Dystrophy (DMD), Multiple Sclerosis (MS), and Acromegaly.

Investments in Antisense Technology LIVER SPLEEN FAT BONE MARROW ASO-KIDNEYS ASO A, 4 µg, n=24, 25d ISIS SMN Rx Increases Survival in a Mouse Model of SMA Improves Muscle Health A K nown Biological T argets Year Genomic Revolution Addition of non-coding RNAs Gene mRNA DISEASE Transcription anslation.

The future applications of antisense technology will solely depend on the performance of novel molecules in the clinical trials. Till date tremendous progress has been made toward the antisense technology, despite the fact this has yet to deliver its full potential. There are still more unanswered questions which need further improvement of Author: Shashwat Sharad.

Extensively revised and updated, Antisense Drug Technology: Principles, Strategies, and Applications, Second Edition reflects the logarithmic progress made in the past four years of oligonucleotide-based therapies, and, in particular, antisense therapeutics and research.

Interpreting lessons learned from the clinical trials of first generation drugs, the book evaluates the technology as a Format: Hardcover. UGCA5’ Antisense RNA. This article gives brief introduction regarding the antisense technology.

Keywords: Antisense technology, protein production, gene expression. INTRODUCTION Diseases are often connected to the insufficient or excess production of certain “Proteins”. If the production of these proteins is disputed many diseases can be File Size: KB.

A unified terminology has not yet been developed to describe technologies in this field. For the purpose of this chapter, we use antisense therapy to refer to the classical antisense approach. Other chapters in this book provide further information and discussions on. Antisense reagents and technology have developed as extraordinarily useful tools for the analysis of gene function.

The capacity of antisense to reduce expression of RNA (including protein-encoding mRNA and noncoding RNA) important in a multitude of diseases has led to the concept of using antisense molecules as drugs to treat those by: 4. Antisense RNA technology Article (PDF Available) in Turkish Journal of Agriculture and Forestry 20(5) January with 3, Reads How we measure 'reads'Author: Kenan Turgut.

Extensively revised and updated, Antisense Drug Technology: Principles, Strategies, and Applications, Second Edition reflects the logarithmic progress made in the past four years of oligonucleotide-based therapies, and, in particular, antisense therapeutics and research.

Interpreting lessons learned from the clinical trials of first generation drugs, the book evaluates the technology as Format: Hardcover. Use of antisense oligonucleotides is a growing field of pharmaceutical and biotech companies and research programs for treatment of several diseases. This book summarizes and presents the best updates, therapeutic principles, m Read more > Order hardcopy Books open for chapter submissions.

Buy Antisense Technology Books online at best prices in India by Wolfgang Nellen,Conrad Lichtenstein from Buy Antisense Technology online of India’s Largest Online Book Store, Only Genuine Products.

Lowest price and Replacement Guarantee. Cash On Delivery Available. Antisense therapeutics is well positioned to play a significant role in the progression of antisense technology for drug development in human diseases.

However, proof of clinical efficacy, of any of the antisense oligonucleotides in the field of oncology, is still missing. Antisense technology prevent the synthesis of specific protein. Antisense technologies are a suite of techniques that, together form a very powerful weapon for studying gene function and for discovering more specific treatments of disease.

Antisense OligonucleotidesWhat are AntisenseOligonucleotides. Antisense technology offers almost unlimited scope for the development of new methods of drug design. It is one of the most approved approaches among several others, for inactivating a single chosen gene.

It has wide applications in crop improvement, treatment of genetic disorders, viral. Summary Extensively revised and updated, Antisense Drug Technology: Principles, Strategies, and Applications, Second Edition reflects the logarithmic progress made in the past four years of oligonucleotide-based therapies, and, in particular, antisense therapeutics and research.

Interpreting lessons learned from the clinical trials of first generation drugs, the book evaluates the technology.Antisense oligonucleotide (ASO) therapy is a groundbreaking concept that has drastically changed the gene therapy landscape.

In the 12 years since the first study was initiated in ALS, ASO therapy has fuelled clinical trials in many different diseases, including two in ALS targeting SOD1 and C9orf72, the two most common genetic causes of ALS.Antisense technology presents an opportunity to manipulate gene expression within the cells to treat various diseases, and acts as a powerful tool for studying gene function utilizing antisense agents to manage the diseases by regulating the expression of the specific factor that actually causes the particular disease.

Highly specific and.