Evaluation of phenyton and benzo[a]pyrene embryotoxicity using inducible nitric oxide synthase (iNOS) knockout mice in embryo culture

by Sonja Kasapinovic in 2001

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  Benzo[a]pyrene (B[a]P), a well-known polyaromatic hydrocarbon, is known for its lung carcinogenicity, however, its role in bladder cancer development is still discussed. Comparative two Cited by: 6. How will Benzo(a)pyrene be Detected in and Removed from My Drinking Water? The regulation for BaP became effective in Between and , EPA required your water supplier to collect water samples every 3 months for one year and analyze them to find out if BaP is present above ppb. If it is present above this level, the system must. Epidemiologic Evaluation of Measurement Data in the Presence of Detection Limits. Jay H. Lubin, Joanne S. Colt, David Camann, Scott Davis, James R. Cerhan, Richard K. Severson, Leslie Bernstein, and Patricia Hartge; Published: 13 September   Evidence has been obtained that a specific isomer of a diol epoxide derivative of benzo(a)pyrene, (+/-)-7 beta,8alpha-dihydroxy-9alpha, 10alpha-epoxy-7,8,9,tetrahydrobenzo(a)pyrene, is an intermediate in the binding of benzo(a)pyrene to RNA in cultured bovine bronchial mucosa. An adduct is formed between position 10 of this derivative and the 2-amino Cited by:

In conclusion, immunotoxicity of B[a]p can be detected using parameters of the immune system such as described in the recently updated OECD guideline. In the present study thymus weight changed and spleen B-cell populations were affected at a dose of 10 mg/kg, a . The uptake, biotransformation, and elimination of {sup 3}H-benzo[a]pyrene (B[a]P) by the gammarid amphipod Leptocheirus plumulosus was evaluated in laboratory exposures. Rapid uptake (i.e. within h) of sediment-associated B[a]P was observed, and during the exposures organism body burdens were. Benzo(e)pyrene appeared to reduce the incidence of carcinomas from 65% (benzo(a)pyrene alone) to 40% (benzo(a)pyrene plus benzo(e)pyrene). However, the incidence of sarcoma in tracheal and peritracheal explants was enhanced two- to three-fold by benzo(e)pyrene given with benzo(a)pyrene compared with benzo(a)pyrene alone. (). Quantification of Benzo[a]pyrene-Guanine Adducts in in vitro Samples by LC Tandem Mass Spectrometry with Stable Isotope Internal Standardization. Journal of Liquid Chromatography & Related Technologies: Vol. 31, No. 20, pp. Cited by: 1.

  A simple analytical procedure was developed for the quantitation of benzo(a)pyrene in human breast milk using solid phase extraction (SPE) combined with high performance liquid chromatography. Before the chromatographic process, SPE, including C18 functional groups in silicagel cartridges, was conducted for sample preparation. A C18 column (× mm id, 3 μm particle size) Cited by: 1. A benzopyrene is an organic compound with the formula C 20 H Structurally speaking, the colorless isomers of benzopyrene are pentacyclic hydrocarbons and are fusion products of pyrene and a phenylene isomeric species of benzopyrene are benzo[]pyrene and the less common benzo[] belong to the chemical class of polycyclic aromatic hydrocarbons. / Formation of benzo[a]pyrene diol epoxide - DNA adducts at specific guanines within K-ras and p53 gene sequences: Stable isotope-labeling mass spectrometry approach. In: Biochemistry. ; Vol. 41, No. pp. Cited by: Benzo(a)pyrene. 7 The Commission receives periodic requests for B(a)P data from the Great Lakes Binational Toxics Strategy B(a)P workgroup. In -7, the Steering Committee Members reviewed reported regional B(a)P emissions in order to improve the regional inventory. This involved.

Evaluation of phenyton and benzo[a]pyrene embryotoxicity using inducible nitric oxide synthase (iNOS) knockout mice in embryo culture by Sonja Kasapinovic Download PDF EPUB FB2

Benzo[a]pyrene (BaP) is a ubiquitous environmental contaminant that is both an endocrine disruptor and a carcinogen. Aromatase (CYP19) is a key enzyme in steroidogenesis that is responsible for conversion of androgens to estrogens and thus plays a key role in steroid by: To gain insight into the toxic effects of the carcinogenic PAH benzo[a]pyrene (BaP) on the typical marine benthic polychaete Perinereis nuntia, we amplified and sequenced genes by creating subtractive cDNA libraries between worms exposed to BaP and solvent assigned functions to the identified sequences and further analyzed the transcriptional profile changes of a set of 50 selected Cited by: Benzo[a]pyrene May BENZO[a]PYRENE.

This is a compilation of abstracts of articles identified during the preliminary toxicological evaluation of evidence on the developmental and reproductive toxic ity of benzo[a]pyrene (BaP, CAS# ).

BaP is a polycyclic aromatic hydrocarbon and a. The peroxynitrite pathway in development: Phenytoin and benzo[a]pyrene embryopathies in inducible nitric oxide synthase knockout mice Article in Free Radical Biology and Medicine 37(11) Nitric oxide generated by nitric oxide synthases (NOSs) can react with reactive oxygen species (ROS), forming peroxynitrite, which may contribute to the ROS-initiated macromolecular damage implicated in the embryopathic effects of both endogenous and drug-enhanced oxidative ble NOS (iNOS) is nonconstitutive in most tissues, and its embryonic expression and developmental importance Cited by: Embryotoxicity of benzo(a)pyrene and some of its synthetic derivatives in Swiss mice Article (PDF Available) in Cancer Research 46(1) February with 34 Reads How we measure 'reads'.

The site of removal has changed over time from predominantly ex situ to in situ remediation methods (Kensa, ). Ex situ bio‐stimulation remediation involves removal and containment of contaminated material which is turned and/or composted to allow aeration and addition of amendments while, when necessary, capturing toxic, volatile intermediates.

This method costs more Cited by: 6. Table LS Summary of the search strategy employed for benzo[a]pyrene Table Evidence pertaining to developmental effects of benzo[a]pyrene in humans Table Evidence pertaining to developmental effects of benzo[a]pyrene in animals after oralFile Size: 3MB.

Benzo[a]pyrene (B[a]P) 1 is a representative polycyclic aromatic hydrocarbon (PAH) that is generated as a result of combustion and is found in significant concentrations in tobacco smoke ().As a ubiquitous environmental contaminant B[a]P is formed as a byproduct of industrialization with traces identified in air- borne particles (), water supplies, as well as food and dietary sources ().


Benzo[a]pyrene, a ubiquitous environmental pollutant, has been suggested to be capable of initiating and/or accelerating atherosclerosis. Accumulation of vascular smooth muscle cells (VSMCs) in vessel intima is a hallmark of atherosclerosis.

Nitric oxide (NO) can suppress VSMCs proliferation and induce VSMCs by: 8. Hardonnière, K. et al. Benzo[a]pyrene-induced nitric oxide production acts as a survival signal targeting mitochondrial membrane potential. Toxicol.

In Vi – ().Cited by: The genotoxic mechanism of action of benzo[a]pyrene involves metabolism to highly reactive species that form covalent adducts to DNA. These anti-benzo[a]pyrene-7,8-diol- 9,oxide-DNA adducts induce mutations in the K-RAS oncogene and the TP53 tumorsuppressor gene in human lung tumors, and in corresponding genes in mouse-lung tumors.

Benzo(a)pyrene‐Induced Changes in p53 and Related Proteins in Mouse Skin. Raisa Serpi. Department of Pharmacology and Toxicology, University of Oulu Cited by: 4. Toxicological Review of Benzo[a]pyrene.

benzo[a]pyrene is complicated. However, some human studies report associations between particular health endpoints and internal measures of exposure, such as benzo[a]pyrene-deoxyribonucleic acid (DNA) adducts, or external measures of benzo[a]pyrene exposure. Overall,File Size: KB.

The application of benzo(a)pyrene (BaP)-toxic equivalent factor to polycyclic aromatic hydrocarbons (PAH) concentrations can provide a more accurate risk assessment from environmental exposure to PAH.

We hypothesized that BaP-equivalent toxicity determined following residential air monitoring among young urban children may vary by by: Addition of benzo[a]‐pyrene (BaP), chrysene, or 7,12‐dimethylbenz[a]anthracene (DMBA) to a synthetic petroleum hydrocarbon mixture of known composition and relatively low embryotoxicity resulted in embryotoxicity that was enhanced or equal to that of crude oil when 10 μl was applied externally to eggs at 72 h of by: Review of Benzo[a]pyrene In Support of Summary Information on the Integrated Risk Information System (IRIS) August This document is a NOTICE.

form of pre‐disseminati ally dissemina on p ted Publ. e by er revi. Comment. w un. This information is distributed solely for the purpose Agency determination or policy. EPA. It does der. Benzo(a)pyrene (B(a)P) is an environmental pollutant which causes various lung toxicities.

The present study was designed to evaluate the protective effects of carvacrol, a monoterpenic phenol against B(a)P-induced lung by: 8. Introduction.

Benzo[a]pyrene (B[a]P), a prototypical and well characterized member of the polycyclic aromatic hydrocarbon (PAH) family (Phillips ; Srivastava et al. ), is a procarcinogen formed in the process of incomplete combustion of organic materials (Gelboin ).Human contact with B[a]P from the environment is unavoidable (Phillips ; Srogi ).Cited by: Benzo(a)pyrene (BaP) or otherwise approved by a peer-review or editorial board.

The peer-review and evaluation system is utilized to protect, maintain, and raise the quality of scholarly material published in serials. benzo(a)pyrene, benzo(b)fluoranthene, benzo(k)fluoranthene, chrysene, dibenz(a,h)anthracene, and indeno(1,2,3-c,d)pyrene. High-performance liquid chromatographic analysis showed that P 1B1 catalyzed benzo[a]pyrene to trans-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene at level of ∼3 nmol min-1 nmol of P only when epoxide hydrolase was present and P 1A1 (with the hydrolase) was able to catalyze benzo[a]pyrene at one-tenth of the activity catalyzed by P 1B1.

The aims of the present study were to compare the toxic effects of benzo[a ]pyrene (Ba P) and to screen for rapid and sensitive biomarkers that can be used to assess the environmental risks of Ba P in earthworms in different natural soil authors exposed Eisenia fetida to 2 types of soil (red soil and fluvo‐aquic soil) spiked with different concentrations (0 mg kg −1, 1 mg kg −1 Cited by: Benzo[a]pyrene (PAH), a polycyclic aromatic hydrocarbon pollutant, is used as a chemical carcinogen in experimental models of cancer.

Carcinogenesis depends on it′s oxidation by CYP1A1 to benzo[a]pyrenediolepoxide, that forms depurinating adducts with DNA that dissociate to form abasic lesions. Benzo[a]pyrene is a ligand for the. be detected in most tissues minutes to hours following exposure.

Benzo[a]Pyrene (BaP) is metabolised by cytochrome P enzymes and epoxide hydrolase resulting in a number of metabolites being formed. These metabolites include the reactive epoxide BaP 7,8 diol-9,epoxide (BPDE), which is believed to be play a role in the carcinogenicity of BaP.

Benzo[a]pyrene is a polycyclic aromatic hydrocarbon and the result of incomplete combustion of organic matter at temperatures between °C ( °F) and °C (1, °F).The ubiquitous compound can be found in coal tar, tobacco smoke and many foods, especially grilled meats.

The substance with the formula C 20 H 12 is one of the benzopyrenes, formed by a benzene ring fused to al formula: C₂₀H₁₂. Benzo(a)pyrene or PAHs: health effects and toxicology Information on benzo(a)pyrene (BaP), an indicator species for polycyclic aromatic hydrocarbons (PAHs), for use in.

Cigarette smoke carcinogens such as benzo[ a ]pyrene are implicated in the development of lung cancer. The distribution of benzo[ a ]pyrene diol epoxide (BPDE) adducts along exons of the P53 gene in BPDE-treated HeLa cells and bronchial epithelial cells was mapped at nucleotide resolution.

Strong and selective adduct formation occurred at guanine positions in codons, and Cited by: Detection of benzo[a]pyrene-DNA adducts in human placenta and umbilical cord blood JP Arnould, P. Verhoest, V. Bach, JP Libert, and J. Belegaud Human & Experimental Toxicology 12, Cited by:   Benzo[a]pyrene (B[a]P) is an increasingly present marine environmental pollutant, yet our understanding of the long-term consequences of reproductive toxicity in marine benthic polychaetes remains limited.

To test the reproductive toxicity of B[a]P on polychaetes, Perinereis nuntia was exposed to B[a]P-contaminated artificial seawater and sexual maturation, the sex ratio, number of eggs Cited by: 4. Relationship between metabolism and bioaccumulation of benzo[α]pyrene in benthic invertebrates benzo[α]pyrene (BαP), was evaluated.

Leptocheirus plumulosus, and Macoma balthica), supporting their use in evaluation of potential PAH impact on the environment. Vol Issue November Pages Cited by: process associated with the action of benzo[alpyrene-diol-epoxideis discussed.

Studies on the chemical and biological properties of (±)-trans-7, 8-dihydroxy-anti-9, epoxy-7,8,9,tetrahydro-benzo[a]pyrene (BP-diolepoxide) suggest that it maybeinti-matelyinvolvedinbenzo[a]pyrene(BP)-inducedcarcinogen-esis.

BP-diolepoxideis. Benzo[a]pyrene-7, 8-diol-9, epoxide (BPDE) is an ultimate carcinogenic product of benzo[a]pyrene (BaP), which causes multiple trophoblast-related diseases. Predominant role of endothelial nitric oxide synthase in vascular endothelial growth factor-induced angiogenesis and vascular permeability.

Sources, exposure and risk evaluation Cited by: 1.